Instructions for Chlamydia trachomatis antibody detection kit (colloidal gold method) (detection of antibodies in secretions)
[Product Name] Chlamydia trachomatis antibody detection kit (colloidal gold method)
[Packing Specifications] 24 servings / box 48 servings / box [Expected Use] For detecting Chlamydia trachomatis antibody (IgM / IgG) in test secretions.
[Principle of Test] Using Chlamydia trachomatis antigen solid phase nitrocellulose membrane, applying the principle of diafiltration indirect method, to detect the antibody of Chlamydia trachomatis in secretion.
[Main components]
Reaction plate 24 parts or 48 parts reagent I 1 bottle of 0.02mol / L PH 7.4 PBS
Reagent Ⅱ 1 bottle of colloidal gold marker [storage conditions and expiration date] The product should be stored at 2 ℃ ~ 8 ℃ conditions, can not be frozen; valid for 24 months.
[Sample requirements]
Draw 1.0ml of physiological saline into the test tube, dissolve the collected secretions in physiological saline, centrifuge, and take the supernatant for detection.
ã€Testing method】
Drop two drops of reagent â… into the central hole of the reaction plate, to be completely infiltrated;
Drop 100µl of supernatant into the well of the reaction plate, to be completely infiltrated;
Add three drops of reagent â…¡ into the well of the reaction plate until it penetrates completely;
Infiltrate three drops of reagent â… into the wells of the reaction plate and wait for complete infiltration.
[Interpretation of results]
Positive: A red round spot appears on the C-end and a red round spot appears on the T-end of the well of the reaction plate, which is positive for C. trachomatis antibody;
Negative: There are red round spots on the C-end and no red round spots on the T-end of the reaction plate well, which is negative for C. trachomatis antibody.
Failure: No red round spots appear on the C-end of the reaction plate well, or no red round spots appear on the C-end and T-end.
[Limitations of inspection methods]
This reagent test is only used to detect Chlamydia trachomatis antibodies rather than direct detection of Chlamydia trachomatis antigen, so a positive result cannot be diagnosed as a Chlamydia trachomatis infection. The diagnosis of the patient's condition should be combined with a comprehensive analysis of the patient's clinical signs and symptoms and test results.
It is possible that secretion samples with low antibody content cannot be detected. Some patients infected with Chlamydia trachomatis do not produce antibodies or produce a small amount of antibodies. At this time, a negative result may be displayed.
When the test result is suspicious, the PCR method is used to confirm the diagnosis.
ã€Product Performance Index】
In-batch precision: both the positive and negative coincidence rates should be ≥95%, and the color depth of the reaction spots should be close.
Precision between batches: Both the positive and negative coincidence rates should be ≥95%.
ã€Precautions】
This product has not yet obtained a product registration number and is for research only, without clinical diagnosis.
Once the test starts operation, it should be carried out continuously according to the operation steps until the end.
When the kit is removed from the refrigerator, the reagents should be brought to room temperature.
There are nice quality and price Paddle board.Accessories including everything you need to enjoy your day on the water including: your board, collapsible aluminum paddle, safety leash , hand pump , waterproof bag for essentials such as cell phone and keys, and an upgraded backpack to hold everything.
Kayak
WEIHAI HITEAM TECHNOLOGY CO.,LTD , https://www.paddleboardmanufacturer.com