Experimental principle of human pituitary adenylate cyclase activating peptide (PACAP) ELISA Kit
English name: Human pituitary adenylate cyclase activating polypeptide, PACAP ELISA
1. The level of human pituitary adenylate cyclase activating peptide (PACAP) ELISA Kit was measured by double antibody sandwich method.
2. First, coat the microplate with purified human pituitary adenylate cyclase activating peptide (PACAP) ELISA Kit antibody to make a solid phase antibody.
3. Then add pituitary adenylate cyclase activating peptide (PACAP) ELISA Kit to the microwells coated with mAb in turn, and then combine with HRP-labeled pituitary adenylate cyclase activating peptide (PACAP) ELISA Kit antibody, An antibody-antigen-enzyme-labeled antibody complex is formed, and after thorough washing, a substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid.
4. The color depth is positively correlated with the pituitary adenylate cyclase activating peptide (PACAP) ELISA Kit in the sample.
5. Finally, the absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human pituitary adenylate cyclase activating peptide (PACAP) ELISA Kit concentration was calculated from the standard curve
.
Objective: To determine the content of pituitary adenylate cyclase activating peptide (PACAP) ELISA Kit in human serum, plasma and related liquid samples.
6. Serum: Blood coagulates naturally at room temperature for 10-20 minutes, centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully and centrifuge again if a precipitate appears during storage. Avoid any cell stimulation during the operation. Use test tubes free of pyrogens and endotoxins.
7. Plasma: EDTA or sodium citrate should be selected as the anticoagulant according to the requirements of the specimen. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm) to remove particles. Collect the supernatant carefully, and centrifuge again if there is any precipitate.
safety:
1. Avoid direct contact with stop solution and substrate. If you accidentally come into contact with these liquids, please rinse them with water as soon as possible.
2. Do not eat, drink, smoke or use cosmetics during the experiment.
3. Do not touch any ingredients in the kit with your mouth.
4 Keep Elisa kit away from children
Bring your own items:
1. 37 ℃ incubator
2. Standard specification microplate reader
3. Precision pipette and disposable tip
4. Distilled water
5. Disposable test tube
6. Absorbent paper
Storage conditions and validity period:
1. Kit storage :; 2-8 ℃.
2. Validity: 6 months
Product Range:
Human, mouse, rat, pig, rabbit, other animal cytokines; apoptosis, active peptides, autoantibodies, thrombosis and hemostasis,
Bone metabolism, liver fibrosis, tumor, hormone endocrine, autoantibody scientific research ELISA test kit
About Us
We are a specialized manufacturer of PP Woven Sacks, Pp Woven Bags, Jumbo Bags , Cement Bags and related products. After years of development, it has become one of the largest production base for bulk bag manufacturing with the annual output of over 800, 000 pieces. The company has solid technical force, advanced processing equipment, strict control system(which has obtained ISO9001: 2000 confirmation and SGS certification)and perfect management. Reputation is the top priority and quality is the first, is the principle of the company.
Our FIBC / PP woven sack / Pp Woven Bags / cement bag / sand bag / pp woven bag / jumbo container bags' specification are,
1) the Pp Woven Bags character are dust-proof, moisture-proof, bear the radiation, safety and substance.
2) Enough intensity on structure, convenient to load and discharge, suit mechanical load and unload.
3) Jumbo Bags Widely used in chemical industry, cement, corn, mineral products and other powdery, granular and massive goods 'packing
4) The safe weight loading (swl)is froom 0.5-3ton, with a cubage froom 500-2300L. The safe factor can be designed to 3: 1, 5: 1, 6: 1 according to the customers'requirements.
5) With the type of tubular and square structure. With single spourt or filling and discharging spourt. Various specifications for one time and rotative use bags to meet different needs.
6) Moisture-proof container bags with PE liner or lamination fabric.
7) Marks such as logos and names of the goods can be printed on the bags on client 'requests
Our Pp Woven Sacks, big bag/ Pp Jumbo Bags /Cement Bags/FIBC jumbo bags /PP big bags can be
1) Any size of Pp Woven Sacks is available
2) UV treated for our Pp Woven Sacks
3) For packing cements, fertilizers, and beans and so on
4) Material: PP fabric coated or not
5) Weight: 150g, 160g, 170g, 186g, 195g for your requirements
6) Color available: Beige, white, yellow
7) Load capacity: 500kg; 1, 000kg; 1, 500kg; 2, 000kg; 2, 500kg; 3, 000kg
Other Bags
Pp Woven Sacks Wholesale,Jumbo Bags,Cement Bags,Promotional Bags
WenZhou JinYuan Packing Manufacture Co.,Ltd , https://www.tote-shoppingbag.com