Reference GB20759-2006 Determination of the Residues of Sixteen Sulfonamides in Livestock and Poultry Meat Liquid Chromatography-Tandem Mass Spectrometry Scope of Application: Applicable to the determination of sulfamethazine, an illegal additive in barbecued pork.
Method principle: The sulfonamide drug residue in the sample is extracted with ethyl acetate, after centrifugation, it is distilled under reduced pressure to near dryness. The residue was dissolved in methanol, acetic acid aqueous solution and n-hexane, centrifugally discarded n-hexane, purified by reverse-phase hydrophilic-philophilic ester equilibrium or cation exchange solid phase extraction cartridge, separated by reverse-phase high-performance liquid chromatography, and quantified by external standard method.
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