How to deal with milk samples in ELISA kit experiment? Method 1: Take 10ml of milk into a centrifuge tube, add 10ml of ethyl acetate, sonicate for 30min, then centrifuge at 4000g for 10min (if an emulsified layer appears between the two phases, place the sample bottle in a water bath at 80 ℃ for about 5min, then Centrifugation) Pipette 1ml of ethyl acetate layer into another test tube, evaporate to dryness under a nitrogen stream at 60 ° C. The residue is buffered with 2ml of buffer solution and used. The pretreatment process takes about 2 hours. ◇◇ For specific content, welcome to enter and click to download for free!
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