Determination of lactose in lactose-free food and beverage by ion chromatography pulse amperometry

An ion chromatography method for the rapid determination of lactose content in lactose-free foods and beverages was established. "Hamilton RCX-30-250 / 4.6 was used as the anion exchange column, NaOH (150 mmol / L) was used as the mobile phase, and pulsed amperometric detection was used. The method has a linear correlation coefficient r of 0.9999, a relative standard deviation of 1.25%, and a sample recovery rate of 94.3% -105.7% when the linear range is 5-50 mg / L. The analysis and display methods for 8 lactose-free foods and beverages The accuracy and precision are good, and the electrochemical detection greatly improves the sensitivity for the determination of low content of lactose. The result proves to be a simple, fast, high sensitivity, and good anti-interference analysis method.

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