There are three steps to establish a solid phase extraction method:
(a) Assemble relevant information;
(b) Establish a preliminary solid phase extraction method;
(c) Optimize the parameters in the method;
The solid phase extraction instrument fully considers the needs of users from the system configuration and method establishment to the establishment of special instructions. The solid-phase extraction instructions included in the software include activation, sample loading, rinsing, drying, and elution, as well as the commonly used liquid handling instructions in the laboratory, which makes the establishment and optimization of the method more convenient.
Common problems of solid phase extraction equipment:
1. Can the solid phase extraction instrument effectively avoid the blockage of the flow path during the extraction process?
Common problems encountered in the application of automatic solid phase extraction equipment: blockage of the flow path causes instrument failure.
When column clogging occurs, most solid phase extraction instruments cannot be automatically detected, causing samples and solvents to leak, causing serious losses and cross-contamination. The flow path of the automatic solid phase extraction instrument is equipped with a highly sensitive pressure sensor. When the flow path pressure is greater than 0.6Mpa, the system will automatically alarm to effectively protect the device.
2. What are the requirements for the processing speed of solid phase extraction?
The solid phase extraction cycle of a single sample is generally about 20 minutes, and the solid phase extraction instruments of general manufacturers are similar. Therefore, in general, the more samples the instrument processes simultaneously, the faster the processing speed. Fully automatic solid phase extraction uses 6 channels + 3 modes for flexible extraction, which maximizes the sample processing speed.
3. Which sampling method is best for the solid phase extraction instrument?
Currently, two methods of positive pressure and negative pressure sampling are commonly used in the market, but negative pressure sampling causes unstable recovery rate and poor instrument repeatability due to the inability to effectively control the flow rate, so mainstream manufacturers generally use positive pressure sampling In this way, the automatic solid phase extraction instrument uses a high-precision syringe pump to positively load the sample to elute the sample, which controls the liquid flow rate more accurately, thereby significantly improving the repeatability of the instrument and ensuring the sample recovery rate.
4. Can the solid phase extraction instrument effectively control cross contamination?
In some automatic solid phase extraction instruments, the sample and solvent use the same pipeline, which is easy to cause pollution. Especially for instruments with no cleaning function, many compounds will remain in the pipeline in a short time, which is the root cause of many experimental failures. . The automatic solid phase extraction instrument adopts a flow path separation unit to automatically select the solvent, and the organic solvent waste liquid has an independent waste liquid channel, which effectively avoids cross contamination.5. How should users choose the appropriate solid phase extraction method?
The easiest way to establish a solid phase extraction method is to search from published standard literature or information provided by SPE column manufacturers. But because the sample matrix may be different, even the target compound is different, the method provided in the literature is not necessarily the most optimized method. Therefore, the extraction parameters in these methods need to be optimized.
Shanghai Haozhuang Instrument Co., Ltd. is a variety of biochemical, light incubator, shaking incubator, constant temperature incubator, mold incubator, hot and cold cycler, shaker, oscillator, stirrer, water bath, centrifuge, electric heating plate. Professional manufacturing enterprise. The company integrates the research, development, production and sales of high-tech experimental instruments. Its products have eight series and more than one hundred varieties. Relying on modern science and technology, the company continuously improves product quality and launches new products to keep it up to the international level. The products are exported to all parts of the country, and have won the trust of users with excellent quality and perfect service. The employees of the company always pursue the enterprise spirit of "unity, truth-seeking and innovation". With the tenet of "customer first, quality first, reputation first", we sincerely welcome new and old customers to visit us for common development and create brilliant! For details, please consult Shanghai Haozhuang Instrument Co., Ltd., and look forward to your inquiry!
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