Shanghai Qiao Yue solid phase extraction steps
The solid phase extraction instrument produced by Shanghai Qiaoyue is a physical extraction process including liquid phase and solid phase. In solid phase extraction, the solid relative separation has a greater adsorption capacity than the solvent that dissolves the separation. As the sample solution passes through the adsorbent bed, the isolate concentrates on its surface and the other sample components pass through the adsorbent bed; high purity and concentrated isolates are obtained by adsorbing the adsorbate only by adsorbing the isolate without adsorbing other sample components.
1. Capacity and selectivity: The capacity of the adsorbent is under optimal conditions, and the amount of unit adsorbent can retain a total amount of strongly retained isolate. The capacity of different bonded silica adsorbents varies widely. Selectivity is the ability of the adsorbent to distinguish between the isolate and other sample matrix compounds, that is, to retain the isolate to remove other sample compounds. A highly selective adsorbent is an adsorbent that retains only the isolate from the sample matrix. Adsorbent selectivity is the function of three parameters: the chemical structure of the isolate, the nature of the adsorbent, and the composition of the sample matrix.
2. Retention and elution: The most common method in a solid phase extraction apparatus is to place the solid adsorbent in a syringe-like column, passing the sample solution through the adsorbent bed, the compound in the sample or through the adsorbent or remaining in On the adsorbent (depending on the relative adsorption of the adsorbent to the solvent). "Retention" is a phenomenon of attraction between the adsorbent and the molecules of the separator, such that when the sample solution passes through the adsorbent bed, the isolate does not move on the adsorbent. Retention is the role of three factors: the separation, the solvent and the adsorbent. Therefore, the retention behavior of a given isolate varies in the presence of different solvents and adsorbents. "Elution" is the process of removing the separation of the separation agent from the adsorbent by adding a solvent that attracts the separation material more strongly than the adsorbent.
3. A brief process of solid phase extraction: one sample includes the isolate and the interferent through the adsorbent; the adsorbent selectively retains the isolate and some interferents, and the other interferent passes through the adsorbent; the adsorbent is rinsed with a suitable solvent, The previously retained interferent is selectively rinsed off and the isolate remains on the adsorbent bed; the purified, concentrated isolate is rinsed from the adsorbent.
Steps:
The operation is slightly different for the difference in the retention mechanism of the filler (the filler retains the target compound or retains impurities). Solid phase extraction operations generally have four steps:
1. Filler retains target compound
Activation - removes impurities from the cartridge and creates a solvent environment.
Load----Dissolve the sample in a solvent, transfer it to the column, and leave the components on the column.
Rinse----Maximize the removal of interferents.
Elution----The substance to be tested is eluted with a small volume of solvent and collected.
2. Filler retains impurities
Solid phase extraction operations generally have three steps:
Activation - removes impurities from the column and creates a solvent environment.
Loading - Transfer the sample into the column, at which point most of the target compound will flow out with the sample base and the impurities will remain on the column, so this step begins to collect
Elution - The components were rinsed off with a small volume of solvent and collected, and the pool was combined.
This is often used to remove pigments in food or pesticide analysis.
Shanghai Qiaoyue specializes in producing all kinds of laboratory instruments. The company's employees have excellent professional quality and experienced technical service team. We have professional senior engineers such as design, development, manufacturing, installation and commissioning of the whole plant turnkey project to serve you. Welcome new and old customers to inquire!
Qiao Yue has served in the field of test equipment for 20 years and has mature experience in the development, design, manufacture and installation of test instruments.
Qiaoyue has long-term cooperation with domestic and foreign research institutes to provide advanced equipment for many domestic and foreign companies.
Qiaoyue focuses on user practice and product development, and has obtained a number of patents and research and development results.
Skin Care,Ordinary Skincare,Face Cleanser,Face Moisturizer
Homesafe , https://www.homesafebio.com